Aqueous Size-Exclusion Chromatography by Paul L. Dubin

By Paul L. Dubin

The quick improvement of latest packings for aqueous size-exclusion chromatography has revolutionized this box. excessive solution non-adsorptive columns now make attainable the effective separation of proteins and the quick and certain decision of the molecular weight distribution of man-made polymers. This know-how is usually being utilized to the separation of small ions, the characterization of associating platforms, and the dimension of branching. even as, basic reports are elucidating the mechanisms of some of the chromatographicprocesses. those advancements in rules and purposes are assembled for the 1st time during this publication. primary concerns are handled: the jobs of pore constitution and macromolecular dimensions, hydrophobic and electrostatic results, and the choice and keep an eye on of column potency. High-performance packings in line with derivatized silica are reviewed intimately. specific strategies are completely defined, together with SEC/LALLS, inverse exclusion chromatography, and frontal sector chromatography.

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7 ) . However, since all the monosaccharides, except ribose, do not increase the solubility of 1-octanol but all increase the solubility of biphenyl having the cavity surface area comparable to that of 1octanol, it follows, as already pointed out, that the cosolvent effect (AFt) of sugars is largely determined by AFint rather than AFcav. Nevertheless, the nP structural modification effect of sugars is important in aqueous gel chromatography because it bears directly on water structure in water-swollen polysaccharide gels.

Oh, D. L. Chum, J. , 267 (1983) 249-265. C. Tanford, P h y s i c a l Chemistry of Macromolecules, Wiley, New York, 1961. C. G i d d i n g s and H. E y r i n g , J. Phys. , 59 (1955) 416-421. W. P. L. Suchan, Separ. , 5 (1970) 259-271. W. P. W. Fleming, J. , P a r t B, 6 (1968) 803-807. W. L. P. Malone, J. , P a r t A-2, 6 (1968) 1349-1355. F. Cassassa, J. Phys. , 75 (1971) 3929-3939. Sephadex F i l t r a t i o n i n Theory and P r a c t i c e , Pharmacia F i n e Chemicals, Uppsala, Sweden, 1979.

52 nd ahom Janado and Yano ( r e f . 27). t i v e Kav value o f each sugar may be regarded r e f l e c t i n g t h e i r d i f f e r e n t i a l i n t e r a c t i o n s w i t h polystyrene. I n t h e present system where a l a r g e pore polystyrene gel (40 8) i s employed, t h e chromatographic r e t e n t i o n w i l l be determined by the combined e f f e c t o f the s t e r i c exclusion and t h e hydrophobic i n t e r a c t i o n s . Since the molecular sizes o f t h e monosaccharides are not g r e a t l y d i f f e r e n t , the s t e r i c exclusion e f f e c t must be n e a r l y the same f o r a l l the sugars.

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